|The Answers to the following questions will be turned in at the beginning of Lab 7.
1. Ingrid has the following SNPs on one copy of chromosome 20:
G at position 334 of gene A
T at position 586 of gene A
C at position 23 of gene B
A at position 437 of gene B
A at position 978 of gene B
Determine if the following combinations of SNPs are considered to be a haplotype only, an allele only, or both.
______________________________ G334, T586
______________________________ C23, A437, A978
______________________________ G334, T586, C23, A437, A978
2. A section of the TAS2R38 gene (spanning the SNP at position 785) was amplified by PCR using DNA from 10 individuals as templates. The PCR products were digested with Fnu4H1 and the digested PCR products were separated by agarose gel electrophoresis. Use the results from the gel below to answer the following questions (a) and (b).
(a) Highlight the correct genotype for each individual.
Individual #1: C785/C785 C785/T785 T785/T785
Individual #2: C785/C785 C785/T785 T785/T785
Individual #3: C785/C785 C785/T785 T785/T785
(b) You are interested in investigating whether the SNP at position 785 of the TAS2R38 gene is associated with susceptibility to sinus infections. Use the gel electrophoresis data (above) and the phenotype data (below) to determine if there is an association between the 785 SNP and sinus infection. You should use the gel electrophoresis and phenotype data to generate a figure that will allow you to address this question.