Supplementary data



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Supplementary Material (ESI) for Perkin Transactions

This journal is © The Royal Society of Chemistry 2001



Supplementary data
The following is step listing for a custom "Begin" procedure for use on an ABI 394 DNA synthesizer which performs an automated nucleoside addition on a synthesis column containing underivatized support. Functions #200, 203, 206, and 209 are custom user defined functions which simultaneously deliver reagents from base positions #7 and #8 to columns 1, 2, 3, and 4, respectively. An acetonitrile solution of nucleoside-3'-O-hydroquinone-O,O'-diacetic acid hemiester (2) and diisopropylethylamine is installed on base position #7. An acetonitrile solution of HBTU/DMAP is installed on base position #8.

- 394 Begin Procedure Listing - (Version 2.00)


Name : Loadl234&CAP 5:29:44P, 3/15/ 1 Steps: 48
Step Function Step Step Active for Bases Safe

Number # Name Time Step

------ ------------------- ----- -------------------------- ----

1 106 Begin Yes

2 64 18 to Waste 5.0 Yes

3 42 18 to Column 30.0 Yes

4 2 Reverse Flush 10.0 Yes

5 42 18 to Column 30.0 Yes

6 2 Reverse Flush 10.0 Yes

7 42 18 to Column 10.0 Yes

8 2 Reverse Flush 5.0 Yes

9 42 18 to Column 10.0 Yes

10 2 Reverse Flush 10.0 Yes

11 I Block Flush 5.0 Yes

12 101 Phos Prep 10.0 Yes

13 200 7+8 to Colm 1 4.0 Yes

14 203 7+8 to Colm 2 4.0 Yes

15 206 7+8 to Colm 3 4.0 Yes

16 209 7+8 to Colm 4 4.0 Yes

17 103 Wait 600.0 Yes

18 2 Reverse Flush 5.0 Yes

19 42 18 to Column 20.0 Yes

20 2 Reverse Flush 10.0 Yes

21 42 18 to Column 20.0 Yes

22 2 Reverse Flush 10.0 Yes

23 42 18 to Column 20.0 Yes

24 2 Reverse Flush 10.0 Yes

25 42 18 to Column 20.0 Yes

26 2 Reverse Flush 10.0 Yes

27 1 Block Flush 5.0 Yes

28 102 Cap Prep 10.0 Yes

29 39 Cap to Column 12.0 Yes

30 103 Wait 100.0 Yes

31 2 Reverse Flush 5.0 Yes

32 102 Cap Prep 10.0 Yes

33 39 Cap to Column 10.0 Yes

34 103 Wait 85.0 Yes

35 2 Reverse Flush 5.0 Yes

36 102 Cap Prep 10.0 Yes

37 39 Cap to Column 10.0 Yes

38 103 Wait 85.0 Yes

39 2 Reverse Flush 5.0 Yes

40 1 Block Flush 3.0 Yes

41 42 18 to Column 20.0 Yes

42 4 Flush to Waste 4.0 Yes

43 42 18 to Column 20.0 Yes

44 4 Flush to Waste 5.0 Yes

45 42 18 to Column 10.0 Yes

46 2 Reverse Flush 10.0 Yes

47 1 Block Flush 4.0 Yes



48 107 End Yes

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