Supplementary data
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Supplementary Material (ESI) for Perkin Transactions This journal is © The Royal Society of Chemistry 2001 Supplementary data The following is step listing for a custom "Begin" procedure for use on an ABI 394 DNA synthesizer which performs an automated nucleoside addition on a synthesis column containing underivatized support. Functions #200, 203, 206, and 209 are custom user defined functions which simultaneously deliver reagents from base positions #7 and #8 to columns 1, 2, 3, and 4, respectively. An acetonitrile solution of nucleoside-3'-O-hydroquinone-O,O'-diacetic acid hemiester (2) and diisopropylethylamine is installed on base position #7. An acetonitrile solution of HBTU/DMAP is installed on base position #8. - 394 Begin Procedure Listing - (Version 2.00) Name : Loadl234&CAP 5:29:44P, 3/15/ 1 Steps: 48 Step Function Step Step Active for Bases Safe Number # Name Time Step ------ ------------------- ----- -------------------------- ---- 1 106 Begin Yes 2 64 18 to Waste 5.0 Yes 3 42 18 to Column 30.0 Yes 4 2 Reverse Flush 10.0 Yes 5 42 18 to Column 30.0 Yes 6 2 Reverse Flush 10.0 Yes 7 42 18 to Column 10.0 Yes 8 2 Reverse Flush 5.0 Yes 9 42 18 to Column 10.0 Yes 10 2 Reverse Flush 10.0 Yes 11 I Block Flush 5.0 Yes 12 101 Phos Prep 10.0 Yes 13 200 7+8 to Colm 1 4.0 Yes 14 203 7+8 to Colm 2 4.0 Yes 15 206 7+8 to Colm 3 4.0 Yes 16 209 7+8 to Colm 4 4.0 Yes 17 103 Wait 600.0 Yes 18 2 Reverse Flush 5.0 Yes 19 42 18 to Column 20.0 Yes 20 2 Reverse Flush 10.0 Yes 21 42 18 to Column 20.0 Yes 22 2 Reverse Flush 10.0 Yes 23 42 18 to Column 20.0 Yes 24 2 Reverse Flush 10.0 Yes 25 42 18 to Column 20.0 Yes 26 2 Reverse Flush 10.0 Yes 27 1 Block Flush 5.0 Yes 28 102 Cap Prep 10.0 Yes 29 39 Cap to Column 12.0 Yes 30 103 Wait 100.0 Yes 31 2 Reverse Flush 5.0 Yes 32 102 Cap Prep 10.0 Yes 33 39 Cap to Column 10.0 Yes 34 103 Wait 85.0 Yes 35 2 Reverse Flush 5.0 Yes 36 102 Cap Prep 10.0 Yes 37 39 Cap to Column 10.0 Yes 38 103 Wait 85.0 Yes 39 2 Reverse Flush 5.0 Yes 40 1 Block Flush 3.0 Yes 41 42 18 to Column 20.0 Yes 42 4 Flush to Waste 4.0 Yes 43 42 18 to Column 20.0 Yes 44 4 Flush to Waste 5.0 Yes 45 42 18 to Column 10.0 Yes 46 2 Reverse Flush 10.0 Yes 47 1 Block Flush 4.0 Yes 48 107 End Yes Kataloq: suppdata suppdata -> Supporting Information 6a suppdata -> Electronic Supplementary Information Yüklə 11.3 Kb. Dostları ilə paylaş:
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