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etermination of growth inhibitory activities of several bioactive compounds of Andrographis paniculata against a panel of human tumor cell lines: 14-deoxy-11, 12-didehydroandrographolide induces a non-apoptotic programmed cell death in T-47D, a breast carcinoma cell line

Tan Mei Lan1, Tengku Sifzizul Tengku Muhammad1, Masanori Kuroyanagi2, Shaida Fariza Sulaiman1, Nazalan Najimudin1
1School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia. 2School of Bioresources, Hiroshima Prefectural University, Shobara-shi, Hiroshima 727 Japan.
The growth inhibitory activities of seven bioactive compounds of the medicinal plant, “Hempedu Bumi” or Andrographis paniculata against a panel of five human tumor cell lines were evaluated. The seven compounds used were andrographolide, 14-deoxyandrographolide, andrographiside, deoxyandrographiside, 14-deoxy-12-methoxyandrographolide and 14-deoxy-11, 12-didehydroandrographolide against Caov-3 (human ovarian carcinoma) cell lines, T-47D (human breast carcinoma) cell lines, Hs-578T (human breast carcinoma) cell lines, Hep G2 (human hepatocarcinoma) cell lines and NCI-H23 (human lung adenocarcinoma) cell lines. Cell survival was carried out using the MTS assay after 72 hours of incubation. Andrographolide, andrographiside, deoxyandrographiside and 14-deoxy-12-methoxyandrographolide appeared to be non-cytotoxic against all the cell lines, as judged by the criterion set by the National Cancer Institute, USA (EC50 was more than 4 µg/ml). However, only 14-deoxyandrographolide and 14-deoxy-11, 12-didehydroandrographolide exhibited prominent cytotoxic activities but was only limited to T-47D cell line (EC50 at 2.800 µg/ml and 1.520 µg/ml, respectively). As principle compounds of A. paniculata, 14-Deoxy-11, 12-didehydroandrographolide appeared to be the most potent as compared to the rest of the compounds. The effects of 14-deoxy-11, 12-didehydroandrographolide on T-47D cells was further confirmed to be non-apoptotic, non-necrotic but programmed in nature as demonstrated using a modified TUNEL assay, trypan blue exclusion assay and annexin V-propidium iodide staining.

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