User Information Plan




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Chemical Analysis Group Lifecycle



User Information Plan





User Information Plan
Document Information:

Filename



Current Owner

Deborah Perkins

Product Identifier

H4033A

Project Identifier

ChemStation


Revision Log:

Date

Reason For Update

March 24, 2006

Initial Revision

April 22, 2007

B.03.01 Release


This product is a four day lecture and laboratory course requiring instruments equipped with diode arrays or VWD’s. This course requires both a student lecture and laboratory manual. You can use either 1100 or 1200 modules.
Notes to Instructor:

B.02/B.03 now use a sequence container. Please read technical note to understand implications. Please familiarize yourself with the SL modules.
General Course Information

H4033A

Course Name

Agilent 1100/1200 Series HPLC 3(D) ChemStation Operation



Course Number Price Duration

H4033A $2417.00 4 days



Description

This course is designed for those who want to enhance their skills in the techniques and software operation of the Agilent 1100 or 1200 Series HPLC 3(D) ChemStation. The course utilizes a lecture format with instrument laboratories. Topics include creating qualitative and quantitative methods, sequencing, diode array optimization, and hardware maintenance.



Outline:

Introduction to the HPLC ChemStation and Windows

Laboratory

Creating and Running an Acquisition Method

Laboratory

Diode Array Optimization

Qualitative Data Analysis

Laboratory

Library Searching

The Diagnosis View and Agilent Diagnotic Tool

Laboratory – Two labs available one for 1100’s and 1200’s one for SL models

Integration

Laboratory

Quantification

Basic Reporting

Laboratory – combined quant and reporting

Creating a Sequence

Laboratory

Sequence Review and Reprocessing

Laboratory

Advanced Reporting

Laboratory

Custom Reporting

Laboratory

OPTIONAL MATERIALS

Commands and Macros

Laboratory

Agilent 1100 Fluorescence Detector


Student Prerequisites

A basic liquid chromatography course such as H1186A, Techniques of HPLC, is required. One month experience on the instrument is desirable. Profile: An analyst or chemist who has responsibility for operation and maintenance of the Agilent Technologies 1100/1200 Series HPLC for routine and non-routine samples. A fundamental understanding of high performance liquid chromatography is expected.


Course File Key

H4033Ast.doc – student lecture manual

H4033Ast.pdf – student lecture manual for printing

H4033Ainst.doc – student laboratory manual

H4033Ainstlab.pdf – student laboratory manual for printing

H40333Ainst.pdf – instructor manual

H4033Alabinst.pdf – instructor laboratory manual

Datafiles.exe – data files for course – you must load data files into their proper directory.

*.ppt files – Lectures

*.doc – individual lecture modules

See course list next page.

Course Files



Course Preparations

Instrumentation Guidelines


  • 2 students per instrument and ChemStation

  • Instruments can be 1100’s, 1200’s, or 1200 SL’s. Should be equipped with solvent cabinet, vacuum degasser, pump (binary, binary SL or quaternary), autosampler or high throughput autosampler, TCC, and DAD.

  • Appropriate Computer with CD drive and printer. Floppy Disk and drive if possible.

  • Chemstation loaded with B.03 G2170AA and G2180AA software
    License #’s 2170 – TL04041172
    2180 - TN040411B9

  • Provide students with administrator log in and password. Lab indicates password is: Agilent.

  • Load course data files and methods into appropriate folders. Do not delete Demo files.



Other Requirements - Lectures

  • High resolution LCD Projector capable of handling 1280 x 1024 resolution.

  • Printed student lecture and lab manual per student.

  • White board and markers for instructor.

  • Instructor requires laptop for lectures.


Other Requirements – Laboratories

  • The following Demo files are required, (no action required on set-up as they load with software)

    • Demo\005-0104.d

    • Demo\Demodad.d

    • Isocra.m and demo sequence

    • Demo\005-0101.D

    • Democal1.M

    • Batch.B and Batch.M

  • Extract Datafiles.exe and load into the following directories:

Chem32\1\Data

  • Integration.d

  • Load the folder 03-15-06 (contains a sequence container)

  • Load the folder 03-20-06 (contains a sequence container)

  • Lab4m.d

Chem32\1\Methods

  • Advrep.M

Note: Customers will build several methods and use acquired data files in this course. If a customer has a problem in one lab and doesn’t successfully create the required .M or .D file, get a copy from another student’s ChemStation.

  • Samples

Four vials are required to complete this course.

Use isocratic test mix part number 01080-68704.


For the high standard: dilute 1 mL of test mix to 10 mL

For medium standard: dilute 3 mL of high standard to 10 mL

For low standard: dilute 1 mL of high standard to 10 mL

For Sample: dilute 5 mL of high standard to 10 mL

Distribute the above into autosampler vials for each instrument. Label the vials:

High Standard

Medium Standard

Low Standard

Sample
Recommended Supplies:


  • Autosampler vials and caps

  • Pasteur Pipettes

  • Pipette bulb

  • Classroom Supplies, markers, flip chart, etc.

  • 3 ½ inch floppy, one per ChemStation

  • HPLC grade water – channel A

  • HPLC grade acetonitrile – Channel B

  • HPLC grade IPA

  • Columns 4.6 X 100 mm C18

  • Column identification module, part # 7982618-564, one per instrument (optional)

  • 1100 Maintenance and Repair CD-ROM, one/Agilent 1100/1200.

  • Safety supplies: disposable nitrile gloves, Safety Glasses, Tyvek lab coat, spill kit, eye wash, universal absorbent wipes, waste labels and Nalgene waste bottles , hood facility for pouring solvents

  • Waste Disposal System

  • Vial Crimper

  • 10 mL graduated cylinder or volumetric flask (suitable for our dilution purposes)

Recommended Spare Parts



Recommended Spare Parts

Agilent 1200

  • Capillaries

  • Spare waste tubing

  • Pump Seals

  • Pistons

  • Frits

  • Solvent inlet filters

  • Rotors

  • Needles

  • Needle Seats

  • Lamps


Tools Required


  • large column wrenches7/16 x ½ and ½ x 9/16

  • small column wrenches ¼ x 5/16

  • pozidrive #1

  • pozidrive #2

  • ZDV union

  • Capillary

  • Small screw driver

  • Flat wrenches 4mm and 14 mm

  • Allen wrench set 1.5 mm 2.5 mm 3 mm 4 mm 5 mm

  • Blanking nut

  • Small beaker

  • Safety glasses

  • Sand paper (small strip)

  • priming tool

  • Agilent 1100 Maintenance and Repair CD-ROM

Typographical error corrections, other corrections, and some slide substitutions were made with the April 2007 revision – these were identified and/or suggested by Ginny Curtis


Additionally, the following edits were made to the course in April 2007:

01_overview:

Added a slide to explain the data organization concept

Added mention of additional paths to the “configure paths” screen

Updated screen capture for Preferences (slide 17). Added instructor notes regarding unique folder creation on or off.


04_qualanal:

Generated new screen capture and notes for slide 3 – Preferences.

Added a 2nd slide on current vs. DA method
07_integration

Added a slide to highlight which method is being saved

Updated instructor notes regarding manual integration and da.m
08_quant

Added a slide to highlight which method is being saved


09_report

Added “which method is being saved” verbage to slide 9


10_sequence

Updated screen capture on Preferences slide.

Added a slide on data organization – what it is and what goes into sequence container

Combined customize sequence table slide and sequence table explicit recalibration (replaced the graphic with add’l columns with graphic of customizing the same list)

Combined Recalibration Settings and Recalibration Report Options slide into one.
10b_sequencereprocessing
Added verbage regarding unique folder creation to slide that outlines reprocessing files generated < b.01.

Combined slides that point out navigation table and ChemStation Explorer interface

Added a slide covering “which method is loaded during data review”

Added a slide showing “view the acquisition method” in data analysis

Added a slide showing “partial reprocessing” in data analysis

Added a slide showing how to update master or sequence method from navigation table


10b_sequencereprocesslab

Added a warning that the sequence container names are similar (classsequence and sequenceclass) and to be certain the correct file is being used.

Changed screen capture for Preferences

Added a few questions about method usage and changing “method to use” from the GUI. Added instructor notes.


Suggested that G2170-90041 (ChemStation data organization guide) should be added to the course as an appendix.



Template Revision T.04

Agilent Technologies Confidential

Printed Copies are Uncontrolled

Page of 10

Date Printed: 3/6/2016

See Project Plan for location of Controlled Documentation





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