Ranunculaceae: Aconitum: A. apoiense; ab038175. Anemone




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Appendix 2. MatK and ITS sequences from Genbank representing 26 genera from Ranunculaceae for molecular clock analysis. Underlined sequences correspond to those used in the age estimation of Aquilegia (Subfamily Thalictroideae).

Ranunculaceae:

Aconitum: A. apoiense; AB038175.

Anemone: A. flaccida; AB110530, A. acutiloba; DQ994677, A. americana; AF542590.

Caltha: C. palustris; AY515232.

Ceratocephala: C. orthoceras DC.; AY954230, C. falcata (L.) Pers.; AY954229.

Clematis: C. villosa; AB110529, C. vitalba; AB110525.

Coptidium: C. pallasii (Schlecht.) Tzvelev; AY954233, C. lapponicum (L.)Tzvelev; AY954234.

Delphinium: D. grandiflorus; AY515249.

Dichocarpum: D. dalzielii (Drumm. & Hutch.)W.T. Wang & Hsiao; EF437129, EF437115; D. sutchuenense (Franch.)W.T. Wang & Hsiao; EF437130, EF437116.

Enemion: E. raddeanum Regel; EF437131, EF437117.

Eranthis: E. stellata; AY515248, E. hyemalis AJ414342.

Ficaria: F. verna Huds.; AY954232, F. kochii (Ledeb.) Iranshar & Rech.f.; AY954231.

Glaucidium: G. palmatum Sieb. & Zucc.; AB069850.

Helleborus: H. multifidus; AY515246, H. tibetanus; AJ414334, H. orientalis; AY515247.

Hepatica: H. nobilis; DQ994672, H. insulares; DQ994673.

Hydrastis: H. canadensis L.; AB069849.

Isopyrum: I. manshuricum Kom.; EF437133, EF437119; I. anemonoides Kar. & Kir.; EF437132, EF437118; I. thalictroides L.; EF437134, EF437120.
Leptopyrum: L. fumarioides (L.) Reichb.; EU827662, EU747249.

Myosurus: M. minimus L.; AJ414344.

Paraquilegia: P. microphylla (Royle) Drumm. & Hutch.; EF437136, EF437122.

Pulsatilla: P. cernua; AB110531.

Ranunculus: R. alpestris L.; AY954221, R. glacialis ; AY954219, R. carpaticola Soó; AY954111, R. garganicus; AY954165, R. polyanthemos; AY954185, R. granatensis; AY954197, R. flammula; AY954204.

Semiaquilegia: S. adoxoides (DC.) Makino; EF437137, EF437123.

Thalictrum: Th. robustus Maxim.; EF437138, EF437125; Th. javanicum Bl.; DQ478615, EF437124; Th. tuberosum L.; EU827661, EU747250.

Trollius: T. riederianus; AY515241, T. japonicus; AY515240, T. chinensis; AY515235.

Urophysa: U. henryi (Oliv.) Ulbr.; EF437139, EF437126.

Xanthorhiza: X. simplicísima Marshall; AB069848.
OUTGROUPS:

Menispermaceae: Cissampelos pareira; EF143858. Cocculus orbiculatus; EF143860. Pericampylus glaucus; EF143869.


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