|Pharmacokinetics and disposition of monoterpene glycosides, derived from Paeonia lactiflora roots (Chishao), after intravenous dosing of antiseptic XueBiJing injection in human subjects and rats
Chen CHENG, Jia-zhen LIN, Li LI, Jun-ling YANG, Wei-wei JIA, Yü-hong HUANG, Fei-fei DU, Feng-qing WANG, Mei-juan LI, Yan-fen LI, Fang XU, Na-ting ZHANG, Olajide E. OLALEYE, Yan SUN, Jian LI, Chang-hai SUN, Gui-ping ZHANG, Chuan LI
Acta Pharmacologica Sinica
Supplementary Information, Appendix S1
Brief Description of Method for Preparation of XueBiJing Injection
XueBiJing injection is prepared from an herb combination of Carthamus tinctorius flowers (Honghua in Chinese), Paeonia lactiflora roots (Chishao), Ligusticum chuanxiong rhizomes (Chuanxiong), Angelica sinensis roots (Danggui), and Salvia miltiorrhiza roots (Danshen). Honghua was first extracted with 30% ethanol and then the extract was diluted with ethanol and centrifuged. The supernatant was reduced followed by dilution with water. After centrifugation, the supernatant was reduced and precipitated with ethanol; after centrifugation again, the supernatant was reduced to yield “H-extract”. Chishao was first extracted with water. The aqueous extract was concentrated under reduced pressure, precipitated with ethanol, and filtered. The filtrate was then reduced and extracted with water-saturated n-butanol. After the n-butanol extract was reduced to dryness, the residual was dissolved in water to yield “C-extract”. Chuanxiong, Danggui, and Danshen were combined and extracted with water. The aqueous extract was treated, in the same way as the preparation of “C-extract”, to yield “CDD-extract”. The preceding H-extract, C-extract, and CDD-extract were combined, diluted with isotonic glucose solution, and fortified with Tween 80. The solution was adjusted to pH 5.8–6.2, ultrafiltered, and sterilized. The finished product of XueBiJing injection was standardized to contain 1.0–1.7 mg/mL concentration of paeoniflorin, 0.2–0.5 mg/mL concentration of hydroxysafflor yellow A, and 0.01–0.20 mg/mL concentration of benzoic acid.
Chemical Structures of the Monoterpene Glycosides Paeoniflorin (5), Oxypaeoniflorin (6), and Albiflorin (4) Present in XueBiJing Injection
MW, molecular mass.