EVALUATION OF LIPID PEROXIDATION AND ANTIOXIDANT STATUS IN SALIVA OF ORAL CANCER –A CASE CONTROL STUDY
Meenakshi Sundari .S1, Revathee. R2 & Uma. R3
1Dept of Biochemistry, Bharathidasan University , Trichy, Tamil Nadu, India 2Dept of Biochemistry, Prist University , Tanjore, Tamil Nadu, India 3Dept of Biochemistry, D.G. Vaishnav College, Arumbakkam, Chennai, Tamil Nadu, India.
The objective of the present study was to evaluate the magnitude of oxidative stress and antioxidant involvement in Oral cancer. Twenty normal healthy subjects in the age group 40 - 60 years served as control ( group I ) and clinically confirmed patients of Oral cancer of the same age served as (group II ) subjects. The following biochemical parameters which includes Lipid peroxides, Glutathione & Protein were assayed in saliva. Antioxidant enzymes which includes Glutathione peroxidase and Catalase, non enzymatic antioxidants such as Ceruloplasmin, Vitamin E & C were determined in saliva. Significantly low levels of Salivary antioxidants such as Ceruloplasmin, and Vitamin E & C were observed in group II subjects when compared to those of controls (p<0.001). However, lipid peroxidation levels was higher & the Protein level was significantly decreased in oral cancer (p<0.001).Glutathione peroxidase was significantly decreased in group II when compared to healthy controls (p<0.01).Catalase activity does not show significant changes in group II subjects. The findings suggest that oxidative stress altered salivary composition in oral cancer subjects there by, reducing enzymatic and non enzymatic antioxidants. The study concludes that the above mentioned parameters would be good bio indicators for diagnosis and prognosis of Oral cancer in saliva which are sensitive, accurate & cost effective.
N.K. Udaya Prakash1, S. Aishwariya2, J. Kotteshwari2, P. Priya Tharshini2,
N.C. Saraswathi2 and S. Bhuvaneswari Udaya Prakash3
1Research and Development, Veltech Dr. RR Dr. SR Technical University Avadi
Alamathy Road, Chennai 600 062.
2 Department of Biotechnology, Veltech Hightech Dr. RR Dr. SR Engineering College,
Avadi Alamathy Road, Chennai 600 062.
3R & D, MARINA LABS, 40, Anna Nedum Pathai, Choolaimedu, Chennai 600 094.
The air we inhale is heavily infested with a wide variety of biological particles in addition to organic substances such as gases, dust and smoke. Airborne fungal spores are one among the biological particles known to be responsible for causing respiratory disorders, in addition to pathogenesis and toxigenesis. This study focus on the presence of Aeromycoflora in the atmosphere over Vellore Corporation, TN. The study on the presence of atmospheric fungi of Chennai, Salem, Madurai, Trichy and Tiruvannamalai belonging to state of Tamil Nadu was previously studied by earlier workers. However, Vellore being a corporation of Tamil Nadu is not studied for its atmospheric presence of fungi so far. Hence, to know the nature of fungi, their no. of presence and their frequency of occurrence the aeromycometric investigation on Vellore Corporation of Tamil Nadu is probed. The study was conducted using the exposure of Petridishes containing Potato Dextrose Agar (PDA). The petridishes were exposed using Andersen Single Stage Sampler (Aerotech Samplers, Inc. Phoenix, AZ, USA). Altogether a total of 19 samples were taken within the limit of Vellore Corporation. The Sampler was operated at the sucking rate of 20 LPM of Air for 5 minutes. The exposed petridish were incubated at room temperature and growing colonies were identified. A total of 28 species belonging to 13 genera were recorded from the atmosphere of Vellore. Among the genera, the genus, Aspergillus was represented by maximum no. of species (10) followed by Penicillium (3 species). The following genera, Cladosporium, Curvularia, Fusarium and Trichoderma were represented by couple of species and all other genera recorded single species each. The details on the colony forming units (CFU) recorded per cubic meter, the percentage contribution, their frequency occurrence and the diversity of fungi will be discussed.
EFFECT OF ETHANOLIC EXTRACT OF MUSA PARADISIACA FLOWERS ON THE KEY ENZYMES OF CARBOHYDRATE METABOLISM STUDIED IN STZ INDUCED EXPERIMENTAL DIABETES IN RATS
C. Shanmuga Sundaram, S. Subramanian
Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600 025, India.
Diabetes mellitus is a metabolic disorder characterized by disturbances in the carbohydrate, protein and lipid metabolism. Though drugs are plenty for the treatment of diabetes, none is found to be ideal due to undesirable side effects. Hence search for novel drugs, especially from plant origin continues. Based on folkloric use, the present study was designed to evaluate the antidiabetic potential of ethanolic extract of the nonfunctional male part of Musa paradisiaca flower (MPF) in STZ-induced experimental diabetes in rats. Qualitative analysis of the extract indicated the presence of biologically active ingredients such as flavonoids and saponins in the MPF extract. Acute toxicity studies revealed the non-toxic nature of MPF extract. Oral treatment of MPF extract (200 mg/kg body weight) to diabetic rats for 30 days established a significant decline in blood glucose and glycosylated hemoglobin levels and a significant increase in plasma insulin level. The altered activities of key enzymes of carbohydrate metabolism such as hexokinase, pyruvate kinase, lactate dehydrogenase, glucose-6-phosphatase, fructose-1, 6-bisphosphatase, glucose-6-phosphate dehydrogenase, glycogen synthase and glycogen phosphorylase in liver and kidney tissues of diabetic rats were significantly reverted to near normalcy by the administration of MPF extract. Thus, MPF extract regulates carbohydrate metabolism by modulating the key regulatory enzymes in the hepatic and renal tissues of diabetic rats. Separation, isolation and identification of the individual components from the extract are under progress.