EVALUATION OF BIOACTIVE COMPOUND AND FREE RADICALS SCAVENGING ACTIVITY OF Strychnos Potatorum Linn.
V.Vijayakumar1 and Dr C.K.Hindhumathy 2
1Department of Pharmaceutical Chemistry, Vinayaka Missions college of Pharmacy, Salem-8
2Department of Biotechnology, V.M.K.V. Engineering College, Vinayaka Missions University, PeriyaSeeragapadi, Salem-636308, Tamil Nadu.
The plants that possess therapeutic properties are exerting beneficial pharmacological effects on the animal body are generally designated as medicinal plants. The plant derived products such as flavonoids, terpenoids and steroids have received considerable attention in recent years due to their diverse pharmacological properties. Recently, medicinal plants constitute and important “National Resource” throughout the world. India is one of the richest plant based ethno medical traditions in the world. Most of the plants are known to possess some principles in their extracts, which have an inhibitory action towards pathogens and to treat many degenerative diseases. Numerous plant constituents have proven to show free radical scavenging or antioxidants activity. In the present study Strychnos potatorum Linn. It is a member of Loganiaceae (Strychnaceae) is herbal medicinal plant , popularly known as Nirmali, is known to act as antiarthritis, antidiabetes, and antihypercholesterolemic activity , hepatoprotective and antiulcer etc. The studies carried to understand the phytochemical constituents and free radical scavenging (antioxidant) properties of Strychnos potatorum Linn., which were assessed by the hydroalcoholic extracts of leaf and seed The preliminary photochemical investigation carried out to in the hydroalcoholic leaf and seed extracts of the plant revealed the presence of alkaloids, flavonoids, phenols, glycosides, steroids, tannins and saponins and the absence of resins. The antioxidant activity was analyzed by DPPH, LPO, H2O2 and nitric oxide radical scavenging assay. Showed that leaf and seed posses the bioactive compounds and excellent antioxidant activity.
OPTIMIZATION STUDIES OF EXPRESSION OF HUMAN SG1 GENE
Lakshmi and C.K.Hindumathy*
*Prof and Dean Department of Biotechnology Vinayaka Mission University, Salem
Biopharmaceutical drugs are mainly recombinant proteins produced by biotechnological tools. The patents of many biopharmaceuticals have expired and bio similar is thus currently being developed. Human SG1 is a hematopoitic cytokine that acts on cells of the neutrophil lineage causing proliferation and differentiation of committed precursor cells and activation of mature neutrophils . Recombinant human SG1 has been produced inb genetically engineered Escherichia coli and successfully used to treat cancer patients suffering from chemotherapy – induced neutropenia. Human SG1 is a 175 a. a. protein containing an extra N- terminal methionine, which is needed for expression in E.Coli. Here we have done the optimization conditions for better production of Human SG1 in a simple and low cost process i.e amenable to scaling up for the production and purification of homogenous and active recombinant human SG1 expressed in E.Coli cells. The “Optimization Studies of Expression of Human SG1 Gene” The Protein shows good Expression in TB media . LB and TB media used for the comparison of the Expression of the Protein. Its shows some variations. i.e In Un induced samples also shows a protein band at 18.6 KD of the protein in TB media, it might be due to leaky expression of the protein by lac uv5promotor and other host cell proteins also more in TB induced medium Because it contains rich nutrients when compared to LB media.The recombinant Human SG1 expression was successfully optimized with Different cell densities (OD’s) and Temperature conditions in two different media, a distinct induced protein band was seen on 12% SDS PAGE Gel with 0.2 and 0.5 mM inducer (IPTG ) concentration at 18.6KDa. Hence, this Human SG1 protein will be used for further physiochemical, Immunological and biological analysis (characterization studies.) and finally will useful to develop therapeutic bioproducts.
PESTICIDE (CHLORPYRIFOS) DEGRADATION BY STRAINS ISOLATED FROM CONTAMINATED SOIL
Gayathri ,V and Hindumathy , C.K *
* Prof and Dean Department of Biotechnology, Vinayaka Mission University, Salem
Today’s modern agriculture produces plentiful food, at a reasonable price, all year round. Most of us take it for granted that we can buy whatever food we want, whenever we want. We rightly expect our food to be safe and nutritious and we have also become used to food, particularly fruit and vegetables, not having any blemishes or other marks. We don’t tend to think about how farmer’s produce food or how it gets from the farm to the shops in “perfect” condition. Over the last 60 years farmers and growers have changed the way they produce food in order to meet the expectations of consumers, supermarkets and governments. In doing so they have made many changes to the way they farm. This often includes the use of “Pesticides”. Farmers use pesticides to protect crops from insect pests, weeds, and fungal diseases while they are growing, to prevent rats, flies, and other insects from contaminating foods while they are being stored and also to safeguard human health, by stopping food crops being contaminated by fungi. However pesticides used to kill unwanted pests, moulds, and weeds, they can also harm people, wildlife and environment. One drawback to this is that pesticides generally kill not only the pest of concern, but also a wide range of other organisms, including beneficial insects and other pest predators. Another drawback to the increasing pesticide consumption use is the development of resistance in pest species. The individual pests that survive pesticide applications continue to breed, gradually producing a population with greater tolerance to the chemicals applied. Keeping the above effect the study was carried out on biological dissipation of pesticide in the Chlorpyrifos contaminated soil. In the present study, the effect of pesticide (Chlorpyrifos) on Rhizospheric soil and Non – Rhizospheric soil of two plants marigold and Canna has been investigated. Further, microorganisms have been isolated from Rhizospheric and Non – Rhizospheric soil, characterized and their pesticide degradation ability was investigated. Most of bio process materials have been taken and analyzed for microbial composition. The efficiency of microbial consortium obtained from each of this bio process material for chlorpyrifos degradation has been studied. Theresult indicates that presence of glucose supports more biomass, which in turn brings about higher degradation and dissipation of pesticide. Maximum 84.5% dissipation was observed through bacterial isolate in presence of glucose as compared to 73.3% dissipation in absence of glucose. In case of fungal isolate 76% dissipation occurred in presence of glucose and only 58% was dissipated in absence of glucose. Both the isolates showed resistance to chlorpyrifos at 10 ppm concentration and also brought about significant dissipation of this pesticide. Therefore, these isolated could be potential candidates for microbe mediated bioremediation of chlorpyrifos contaminated soils.