ANEMIA IN FOUR YEARS OLD CHILDREN OF KATTANKULATHUR AREA.
Department of Biochemistry SRM Medical college Hospital and Research centre SRM University Potheri
Anemia is a serious concern for young children because it can result in impaired cognitive performance, behavioral and motor development, co-ordination, language development as well as increased morbidity from infectious disease.The aim of this study was to assess the prevalence of anemia and its relation to variables such as age, gender, weight, height, mother’s education, total family numbers, Birth order, number of children in the family and socio economic factor in toddlers of Kattankulathur area. The study was approved by institutional ethics committee of SRM Medical college Hospital and Research centre. A total of 116 children in the age of 4 years were included in this study. Written informed consent was obtained from the parents or guardians of all child participants. The age of the children was recorded from the date of birth written in the vaccination card. The information regarding birth order of child, number of siblings, total family members, parents’ education, employment and socioeconomic status was collected through an interview. Weight and Height of the children were taken for assessing their nutritional status. Blood hemoglobin levels of children were estimated by cyanmethemoglobin method, the blood sample obtained by finger prick method. WHO standards for this age group were used to define the presence and severity of Anemia.Data were analyzed by the statistical package SPSS version 17. Chi square was used and p value < 0.05 was considered as statistically significant.The prevalence of anemia was 63.8 %. The percentage of anemic children among male and female was 31.0 % and 32.7 % respectively. BMI, total family members, mothers’ education and socio economic factor were important determinants of anemia. There was no significant relationship between anemia and birth order of child and number of siblings. The prevalence of anemia in the children was high 63.8 %. All the malnourished children should be screened for anemia.
EVALUATION OF PHARMACOGNOSTIC, PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL STUDIES ON Ruellia tuberosa L. (WHOLE PLANT).
B Arirudran,1, A Saraswathy1, Vijayalakshmi Krishnamurthy2
1Captain Srinivasa Murti Drug Research Institute for Ayurveda (CCRAS), Anna Hospital Campus, Arumbakkam, Chennai, India-106.
2Associate Professor Department of Biochemistry, Bharathi Women’s college affiliated to University of Madras, Chennai, India-108.
Ruellia tuberoa L. of Acanthaceae family is a Minnie root medicinal, tropical plant widely distributed in south East Asia. In folk medicine, it has been used as diuretic, antidiabetic, antipyretic, analgesic, anti-hypersensitive, thirst-quenching, and antidotal agent. Recently this plant has been incorporated as a component in an herbal drink in Taiwan. However a very few chemical constituents and pharmacological activities have been reported for this species. As there is no pharmacognostic work reported on whole plant, the present investigation attempts to study the pharmacognostical, preliminary phytochemical studies along with HPTLC, fingerprinting analysis and antimicrobial activity of successive extraction using n-hexane, chloroform, ethyl acetate, alcohol and separate aqueous extract from whole plant of Ruellia tuberosa L. against different bacterial and fungal organisms (ATCC, MTCC) using disc diffusion method. Preliminary organic analysis revealed the presence of tannin, flavonoid, steroid, triterpnoid and phenol in different extracts respectively. Physiochemical studies revealed that total ash is 13.53%, acid insoluble ash is 2.36%, alcohol soluble extractive value is 7.67%, water soluble extractive value is 24.78% and loss on drying at 105°C is 11.29%. The antimicrobial study revealed that the chloroform, ethyl acetate, alcohol and aqueous extracts were active against all the bacteria tested and showed significant antibacterial properties. The aqueous extract exhibited less activity against fungal organisms. These specific identities will be useful in identification, authentication of the raw drug and pharmacological activities associated with traditional folk remedy. Thus it may be informed that Ruellia tuberosa L. may be used to treat oral bacterial diseases.
INSILICO STUDIES ON THE INHIBITORY EFFECT OF FLAVONOIDS ON ANGIOTENSIN CONVERTING ENZYME ACTIVITY
Department of Biochemistry, Meenakshi College for women, Chennai, India
Angiotensin converting enzyme is an exopeptidase that participates in the body’s renin-angiotensin system. By catalyzing the conversion of angiotensin I to angiotensin II and degradation of bradykinin, it results in vasoconstriction and hypertension. It is one of the key enzymes involved in cardiovascular, renal and diabetic disorders and thus can act as a potential drug target. Ligands that can block its activity can be applied for designing novel drugs. Flavonoids are polyphenolic secondary metabolites produced by plants. They are naturally available in vegetables and fruits. This study involves insilico interaction analysis of the drug target with flavonoid ligands obtained from vegetables. Computational Biology tools are harnessed for the structural analysis of the target, docking studies and toxicity prediction of the ligands. The results indicate that flavonoids seem to be natural, safe and potent inhibitors of Angiotensin converting enzyme and further facilitate the understanding of their probable mechanism and application in inhibitor based therapy.
GREEN SYNTHESIS AND APPLICATIONS OF SILVER NANOPARTICLES FROM MEDICINAL PLANT
Jancy Mary.E, ,Inbathamizh.L M.Sc., M.Phil
Department of Biochemistry, Meenakshi College for Women, Chennai, India. email@example.com
The environmental friendly synthesis of nanoparticles is a revolutionary step in the field of nanotechnology. In this study, the green synthesis of silver nanoparticles was carried out using rare medicinal plant leaf extract as reducing agent. UV–visible spectroscopy was used for quantification of silver nanoparticle synthesis. The synthesized silver nanoparticles were characterized with Scanning electron microscopy (SEM), Transmission electron microscopy analysis (TEM), X-ray Diffraction (XRD) and Fourier transform Infrared Spectroscopy (FTIR). The invitro antioxidant properties of the green synthesized silver nanoparticles were evaluated by various antioxidant assays. The findings of the present study suggested that the nanoparticle from this plant could be a potential natural source of antioxidants and could have greater importance as a therapeutic agent in preventing or slowing oxidative stress related degenerative diseases, such as cancer. The Anticancer effects of silver nanoparticles from the medicinal plant on HEP 2(Human Epithelium cells of laryngeal cancer) and normal Vero (Verda Reno) Cell lines were evaluated by MTT (3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide) assay. The greater activity of the nanoparticles on HEP 2 cell line suggested further application of these nanoparticles as anticancer agents.