BIOCHEMICAL, HEMATOLOGICAL AND HISTOLOGICAL EFFECT OF MALAYSIAN BUAH NAGA FRUIT EXTRACT ON HEPATOPROTECTIVE ACTIVITY IN PARACETAMOL–INDUCED EXPERIMENTAL ALBINO RATS
Mainul Haque1 MBBS, MPhil, MSc (UK), CIDTT (UK), U.S.Mahadeva Rao2,
1Professor,email@example.com / firstname.lastname@example.org
2Associate Professor,UniSZA, Malaysia
Preliminary studies on the effects of dietary supplementation with Buah naga or Red pitaya fruit on some biochemical, and haematological parameters and histological examinations of liver, were investigated in albino rats in which liver damage was induced by paracetamol (PAM). Thirty six rats were divided into six groups (including hepatotoxic and non – hepatotoxic), and were fed with 5 and 10% Buah naga fruit extract supplemented diet. Weight changes and parameters including alanine transaminase(ALT), aspartate transaminase(AST), total protein, glucose, total triglyceride(TG), total cholesterol, reduced glutathione(GSH), lipid peroxidation(LPO), packed cell volume(PCV), total and differential leucocyte count were determined using standard methods. The consumption of a Buah naga supplemented diet change the weight of the animals as well as alteration in the levels of glucose, protein, ALT and AST in the hepatotoxic groups towards normalcy. The GSH level was significantly increased (p<0.05) while TG was reduced in hepatotoxic group fed extract supplemented diet. Glucose was significantly reduced to near normal (p<0.05) in the two treated groups. Similar results were observed in cholesterol and LPO status. WBC, Hb and PCV were significantly reduced in hepatotoxic groups and refurbished in treated animals. The hepatotoxic control had significant reduction in neutrophil count and recuperated to near normal in treated rats. Histological Studies: The necrotic effects of paracetamol seen in the abnormal histological changes are gradually regenerated to its native architecture in the hepatotoxic treated groups. Thus the present study conjectured that Buah naga consumption prevent or treat the PAM induced hepatotoxicity and associated other deleterious effects.
An indigenous water treatment method uses Moringa seeds in the form of a water-soluble extract in suspension, resulting in an effective natural clarification agent for highly turbid and untreated pathogenic surface water. Efficient reduction (80.0% to 99.5%) of high turbidity produces an aesthetically clear supernatant, concurrently accompanied by 90.00% to 99.99% bacterial reduction. Application of this low-cost treatment with Moringa is recommended for simplified, point-of-use, cheap and easy method for developing countries, especially at household level.
ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITY OF METHANOLIC EXTRACT OF EUPATORIUM TRIPLINERVE VAHL.
Anusha Bhaskar1, Lalit R. Samant1, G. Selvamangai2
1 Department of Biochemistry, Centre for Research and Development, PRIST University,
2 Department of Biotechnology, Alpha Arts and Science College, Chennai.
Plants synthesise and preserve a variety of biochemical products, many of which are extractable and used as chemical feed stocks or as raw material for various scientific investigations and industrial utilization. Many of the industrially and commercially used pharmaceuticals are products of secondary metabolism in microbial or plant systems. Eupatorium triplinerve Vahl. is an erect annual herb, grows up to 90 cm in height. Leaves simple, opposite, lanceolate, acuminate, glabrous, subsessile; flowers light blue, tubular coryms; fruits 5 sided truncated. The plant shows various medicinal properties against diseases viz. hemorrhage, hemoptysis, menorrhagia, wounds, edema, ulcers, stomatitis, cardiac debility, skin diseases, poison bites, cough, asthma, bronchitis and general debility etc. An attempt has been made to explore the free radical scavenging property of the leaf extract of the plant and isolate a novel compound responsible for it. The methanol extract of the Eupatorium triplinerve Vahl was assessed for antioxidant activity using a series of well-established assays such as In vitro DPPH radical scavenging activity, NO and the H2O2 scavenging and FRAP. The phytochemical analysis with different extraction solvents viz. methanol, petroleum ether and water produce significant findings. The total phenolic and flavonoid contents were estimated. The various antioxidant activity was compared with standard antioxidants such as BHT, Ascorbic acid. Since the ROS are the serious threat and produces ailments such as cancer; further studies are going on to observe cytotoxicity of the plant extract on the cancer line cell. The antioxidant property of the extract of E. triplinerve as observed in the present study might be useful for the development of newer and more potent antioxidants. The present abstract of E. triplinerve Vahl. is an attempt to provide a direction for further research.
HEPATOPROTECTIVE ACTIVITY OF CASSIA AURICULATA (LINN.)
A.S.Sheeba Malar1, K. Revathi1, D.Anandhi2
1Dept. of Zoology, Ethiraj College for Women,
2Dept of Biochemistry, DG Vaishnav College.
Alcohol toxicity is one of the world’s major health problems. Significant numbers of people are affected due to severe fatal diseases caused by alcohol consumption. Herbal medicines are traditionally used to help alcoholism and other addictions. Various species of Cassia are rich in anthroquinones and bioflavonoids which are hypocholesterolemic and hypolipidemic agents that maintain a balanced cholesterol ratio. A number of hepatotoxic agents cause accumulation of fatty acids deposits predominantly triglycerides in the liver. In the present study the accumulation of triglycerides in ethanol treated rat liver. It may be an imbalance between the rate of synthesis and the rate of release of triglycerides by the parenchyma cells of hepatocytes into the systemic circulation. Considering the enzyme alterations, the levels of cholesterol ester hydrolase, cholesterol ester synthase, Lecithin cholesterol acyltransferase and Lipoprotein lipase were found to be significantly increased in liver of rats treated with ethanol. Cholesterol esters have been reported to influence membrane permeability. Oral administration of Chrysophanol significantly altered the levels of cholesterol ester hydrolase, cholesterol ester synthase, and lecithin cholesterol acyltransferase and lipoprotein lipase near to control. The present investigation emphasizes the usefulness of C.auriculata in traditional medicine as a hepatoprotective activity Chrysophanol against ethanol induced hepato toxicity.