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ICBTMM-039




SYNTHESIS, CHARACTERISATION AND BIOLOGICAL EVALUATION OF NOVEL COUMARIN DERIVATIVES




K. HemanthSudheer Kumar*1, P. Ramalakshmi1, C.N. Nalini1, P. Selvam1, Devi.R1 ,

R. Sivamani1, and S. Arun Kumar2.


1Department of Pharmaceutical Chemistry, C.L.BaidMetha College of Pharmacy, Chennai-97.

2Department of Pharmaceutical Chemistry, S.R.M.College of Pharmacy, Chennai-89.
Coumarin chemically known as 2H-1-benzopyran-2-one. It has a significant resemblance to the construction of Vit-k and this led to assumptions that the substance functions against prothrombin that leads to blood clotting. They have varied bioactivities such as inhibition of platelet aggregation, anti-inflammatory2, anti-convusant3, anti-viral4, anticoagulant5, antioxidant6, antitubercular8, antifungal9, anti-HIV10, anticarcinogenic material11 and antihistamine. The purity of the compounds was checked by TLC on pre-coated SiO2 gel (HF254 200 mesh) aluminum plates (E. Merck) using Dichloromethane: Ammonia: Methanol as eluent and visualized in UV-chamber. For Biological evaluation sources are swiss albino mice and method is Hot plate. For evaluation of acute oral toxicity following procedures are OECD Guidelines-423. Evaluation of Invitro anti-microbial activity is by Paper Disc Diffusion Method for knowing Preliminary screening of anti-microbial activity. Among all the title compounds [IIa-1 (Ciprofloxacin)], [IIa-2 (Norfloxacin)], [IIa-3 (Sparfloxacin)], [IIa-5 (n-Methyl piperazine)], [IIb-2 (p-toludine)], [IIb-5 (P-anisidine)], [IIb-6 (Sulphanilamide)], [IIb-7 (sulphadiazine)], [IIb-8 (Sulphamethoxazole)], [IIc-2 (tyrosine)], [IIc-3 (Tryptophan)] synthesized mannich bases with prilimary and secondary amine substitutions showed moderate to good anti oxidant activity. The importance of coumarin ring with the features of substitutes of piperazine, sulpha, aniline and amino acids responsible for the analgesic, anti inflammatory, anti microbial, anti tubercular and anti oxidant activities and therefore may serve as a lead molecule for further modification to obtain clinically useful novel entities in the new millennium

ICBTMM-040




SCREENING AND IDENTIFICATION OF TAXOL PRODUCING ENDOPHYTIC FUNGI FROM ENDANGERED MEDICINAL PLANT

Nomila Merlin J1, Nimal Christhudas I.V.S2, Praveen Kumar P2, Agastian P2


1Department of Biochemistry, Annai veilankanni’s College, Saidapet,Chennai 600 015,

2Department of Plant Biology and Biotechnology, Loyola College,Chennai – 600 034.


An endophyte is an endosymbiont, often a bacterium or fungus. It lives within a plant for at least part of its life without causing apparent disease. In this study, 25 endophytic fungal isolates were obtained from root of Tylophora indica (Burm.f) and screened for presence of taxol. Among the 25 isolates, Fusarium solani LCPANCF01 was identified based on the micro morphology, cultural characteristics and sequence analysis by using internal transcribed spacer (ITS1 & ITS4). The sequence was submitted to GenBank (JN786598). The F.solani LCPANCF01 strain was grown in M1D liquid medium for 21days and it was extracted by using dichloromethane. The presence of taxol was confirmed by using TLC, UV, IR, HPLC and ESI-Mass spectroscopy analysis by comparing with standard drug. The amount of taxol was quantified as 247μg/L in HPLC. The isolated fungal taxol was screened for anticancer activity by in vitro cytotoxicity assay using VERO and HeLa cell lines. The results suggest that the endophytic fungi present in T.indica, showed anticancer activity. The discovery of such microbiological production of drug can revolutionize the search for effective pharmaceutical agents to control cancer.

ICBTMM-041




BIOCHEMICAL CHANGES AND ANTIBACTERIAL STUDY OF FUMES ON PATHOGENIC MICROORGANISMS

Mrs. S.Vaidehi 1, MS. G.Sridevi2


1. Assistant Professor, Department of Biochemistry, D.G Vaishnav College, Chennai-106

2. Research Scholar, Department Of Biochemistry, Mohammad Sathak College, Chennai.

sridevigangadharan@gmail.com
The lifestyle of people in this changing world has intensified the quantum of pollution paving way to new diseases. Microbial pollution is the most important type of pollution for personnel working in laboratories, hospitals, industries of food and beverages. The widely adopted method of fumigation in microbial labs, hospitals is the usage of formaldehyde with potassium permanganate. Frequent usage of formaldehyde can lead to sulphydryl poisoning, protein denaturation leading to cancer. The smoke of medicinal plants can be used as a potential alternative. In this study four common pathogenic organisms in the environment namely Klebsiella pneumoniae, Pseudomonas aeuroginosa, Staphylococcus aureus, Escherichia coli were exposed to fumes of Sambrani, Incense stick and Dharbai to look for antibacterial activity and changes , if any, on their structural compositions like total protein, membrane protein, phospholipid before and after exposure to fumes. The results showed that fumes of sambrani completely inhibited the growth of K.pneumoniae and S.aureus, showing a drastic decrease in the amount of total protein in K.pneumoniae, E.coli. The concentration of total protein, membrane protein was also found to be decreased in other organisms after exposure to fumes of dharbai, incense stick. The amount of phospholipids in all the organisms was found to be more after exposure to fumes. From this study it can be concluded that exposure to fumes of sambrani, incense sticks, dharbai shows antibacterial activity and changes in biochemical parameters on pathogens. Thus the fumes can be recommended as fumigant.

ICBTMM-042




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