|NELLIE CHIYE SUGII
Harold L. Lyon Arboretum
University of Hawaii-Manoa Phone: (808)-988-0470
3860 Manoa Road e-mail: firstname.lastname@example.org
Honolulu, HI 96822
M.S., Horticulture Science: University of Hawaii, Manoa
M.S., Horticulture Science: University of Hawaii, Manoa
1998 to present: Assistant Researcher, Harold L. Lyon Arboretum, University of Hawaii
Manager of the micropropagation laboratory focusing primarily on the tissue culture of native
Hawaiian plants and tropicals. Curator of Native Hawaiian Plants.
1988 to 1998: Research Associate III, University of Hawaii, Dept. of Horticulture
Conducted Dendrobium orchid and Anthurium research for crop improvement utilizing classical
breeding techniques as well as genetic engineering. Main duties included: tissue culture (initiation,
regeneration and maintenance of in vitro plant cultures), plant transformation, and molecular analysis.
General lab management included ordering of chemicals and supplies, supervision of students and
1985-1988: Sales/Research Manager, Hawaiian Floral Nursery, Waimanalo, Hawaii
Responsible for plant sales to local, mainland and foreign clientele. Supervision of workers, planting
and shipping schedules, and obtained in vitro plant material for propagation from local and out of state
suppliers. General nursery duties included setting pesticide and fertilizer spray and watering
schedules, inventory and book keeping. Initiated their orchid breeding program.
1982 to 1985: Laboratory Technician, H&R Nurseries, Waimanalo, Hawaii
Clonal and seed in vito propagation of orchid plants. Helped to initiate their R&D program for
improvement in orchid breeding.
1981-1982: Graduate Assistant, University of Hawaii, Dept. of Horticulture
Nursery propagation, data recording and analysis of orchid and anthurium plants grown for selection.
Lab duties included in vitro plant propagation through seed germination and/or cloning, culture media
preparation, maintenance of cultures. Supervised student help.
March 1981 to October 1981: Student Help, University of Hawaii, Dept. of Horticulture
1980 to 1981: Clerical/Nursery Worker, University of Hawaii, Facilities Management
June 1980 to August 1980: Nursery Worker, Oumae Nurseries, Kaimuki, Hawaii
1978 to 1980: Ward Clerk, Straub Clinic and Hospital Inc., Honolulu, Hawaii
Sugii, N.C. 2003. Flaming Fabaceae:using an alcohol flame to break seed dormancy. Native Plant Journ.
Sugii, N.C. and C. Lamoureux. In press. Tissue culture as a conservation method: an empirical view from
Hawaii. In: Guerrant, E., Haven, K. and M. Maunder (eds) Saving the Pieces:The value, limits and practice of off-site plant conservation in support of wild diversity. Chicago, IL.
Kuehnle, A.R., F.C. Chen and N.C. Sugii. 2001. Transgenic Anthurium, p.3-15. In: Bajaj, Y.P.S. (ed.).
Biotechnology in agriculture and forestry, Vol. 48, Transgenic crops. Springer Verlag, Berlin
Sugii, N.C. and C. Lamoureux. 1998. Micropropagation: an important tool in the conservation of
endangered Hawaiian plants. In: Rose, R. and D. Haase (eds) Symposium Proceedings, Native Plants:
Propagating and Planting. Oregon State University, Oregon, pp 43-48
Chen, F.C., Kuehnle, A.R. and N. Sugii. 1997. Anthurium roots for micropropagation and Agrobacterium
tumefaciens-mediated gene transfer. Plant Cell, Tissue and Organ Culture 49:71-74
Kamemoto, H., Amore, T.D., Kuanprasert, N., Kuehnle, A.R. and N. Sugii. 1997.Evaluation of
Dendrobium crosses involving four new amphidiploid parents.Univ. of Hawaii CTAHR Research Ext.
Kuehnle, A.R., N. Sugii, F.C. Chen, N. Kuanprasert, R. Fukui, and A. Alvarez. 1996. Peptide biocides for
engineering bacterial blight resistance to floral Anthurium. In Vitro Cell. Dev. Biol. 32:72A.
Kuehnle, A.R., Halloran, J., Kamemoto, H., Amore,T., Lichty, J. and N. Sugii. 1996. Anthurium cut
flower breeding and economics. Univ. of Hawaii CTAHR Research Ext. Series 165:1-6
Amore, T.D., Kamemoto, H., Kuehnle, A.R. and N. Sugii. 1996. Rejuvenation of virused amphidiploid
Dendrobium breeding plants. Hort. Digest 110:3-4
Kuehnle, A.R., Kamemoto, H., Rauch, F., Lichty, J., Amore, T.D. and N. Sugii. 1996.Anthurium cultivars
for container production. Hort. Digest 108:1-4
Barry, K., Hu, J.S., Kuehnle, A.R. and N. Sugii. 1996. Sequence analysis and detection using
immunocapture-PCR of Cymbidium mosaic virus and Odontoglossum ringspot virus in Hawaiian
orchids. Journal Phytopathology 114:179-186
Kuehnle, A.R., Chen, F.C. and N. Sugii. 1995. Novel approaches for genetic resistance to bacterial
pathogens in flower crops. HortScience 30:456-461
Kamemoto, H., Kuehnle, A.R., Amore, T.D. and N. Sugii. 1993. Evaluation of PPPC-type dendrobium
progenies. Hort. Digest 99:4-9
Kuehnle, A.R. and N. Sugii. 1993. Use of biotechnology in breeding Dendrobium orchid. In: Ichihashi, S.
and Kimura, T (eds) Proceedings of the Nagoya International Orchid Show ’93. The Organizing
Committee of the Nagoya International Orchid Show ’93, Aichi-ken, Japan, pp52-54
Kuehnle, A.R. and N. Sugii. 1992. Update on somatic embryogenesis research. In: Delate, K.M. and
Tome, C.H.M. (eds) Proceedings of the Fifth Hawaii Anthurium Industry Conference, HITHHR
02.02.94, University of Hawaii, Honolulu, pp15-16
Kuehnle, A.R., Chen, F.C. and N. Sugii. 1992. Somatic embryogenesis and plant regeneration in
Anthurium andraeanum hybrids. Plant Cell Reports 11:438-442
Kuehnle, A.R. and N. Sugii. 1992. Transformation of Dendrobium orchid using particle bombardment of
protocorms. Plant Cell Reports 11:484-488
Kuehnle, A.R. and N. Sugii. 1991. Callus induction and plantlet regeneration in tissue cultures of
Hawaiian anthurium. HortScience 26(7):919-921
Kuehnle, A.R. and N. Sugii. 1991. Induction of tumors in Anthurium andraeanum by Agrobacterium
tumefaciens. HortScience 26(10):1325-1328
McConnell,J. and N. Tomomitsu. 1983. Kappa carrageenan as an agar substitution for transflasking
Dendrobium seedlings. American Orchid Society 52(11):1174-1177
Kamemoto, H., Kuehnle, A.R., Amore, T.D. and N. Sugii. 1996. Dendrobium Miyoko Azuma and
Dendrobium Sharon Sewake-Seed propagated potted plant cultivars. Hort. Digest 108:5-9
Kamemoto, H., Kuehnle, A.R., Amore, T.D. and N. Sugii. 1995. Dendrobium Uniwai Royale, UH1081.
Hort. Digest 105:1-4
Kamemoto, H., Kuehnle, A.R., Amore, T.D. and N. Sugii. 1995. Dendrobium Lim Chong Min, UH1382.
Hort. Digest 105:5-6
Kamemoto, H., Kuehnle, A.R., Amore, T.D. and N. Sugii. 1995. Dendrobium Louis Bleriot, UH1392.
Hort. Digest 105:7-8
Kamemoto, H., Kuehnle, A.R., Amore, T.D. and N. Sugii. 1994. Dendrobium Betty Nakada and
Dendrobium Remy Hartmann-Two new seed-propagated potted plant cultivars. Univ. of Hawaii
Hort. Digest 101:1-4
Kamemoto, H., Kuehnle, A.R., Amore, T.D. and N. Sugii. 1993. Dendrobium ‘Alii’ and ‘Rudolph’
Anthurium. Hort. Digest 99:1-9
Kamemoto, H., Kuehnle, A.R., Amore, T.D. and N. Sugii. 1993. Dendrobium Pua`ala, UH1182. Hort.
Kamemoto, H., Kuehnle, A.R., Amore, T.D. and N. Sugii. 1991. Dendrobium Mini Pearl ‘Elaine Fuchigami
4N’. Hort. Digest 94:5-6
Kamemoto, H., Kuehnle, A.R., Amore, T.D. and N. Sugii. 1991. UH1002 and UH1041 Dendrobium.
Hort. Digest 95:6-7
Kamemoto, H., Amore, T.D. and N. Sugii. 1990. Dendrobium Susan Takahashi, UH999-A seed
Propagated flowering potted plant cultivar. Hort. Digest 90:5-7
Kamemoto, H., Amore, T.D. and N. Sugii. 1990. Dendrobium Sylvia Yuen. Hort. Digest 91:1-4
Honors and Awards
2001 Poster: Hawaii Conservation Conference 2001, Hilo, Hawaii
2000 Session speaker: Hawaii Conservation Conference 2000, Honolulu, Hawaii
2000 Session speaker: World Botanic Gardens Congress 2000, Asheville, North Carolina
Invited speaker: Ex Situ Plant Conservation Symposium, Chicago, Illinois
1999 Poster: Hawaii Conservation Conference 1999, Honolulu, Hawaii
Invited speaker: Native Plants:Propagation and Planting, University of Oregon, Corvallis,
Nagoya International Orchid Congress Grand Prize for best scientific paper and poster
(shared with Dr. A.R. Kuehnle), Nagoya, Japan
Invited speaker: Nagoya International Orchid Congress 1993
1979 Agricultural Merit Award, University of Hawaii, Manoa
Hawaii Rare Plant Restoration Group(HRPRG)
HRPRG Oahu Steering Committee
Hawaii and Pacific Plants Recovery Coordinating Committee(HPPRCC)
International Plant Propagators Society (IPPS)
American Association of Botanical Gardens and Aboreta (AABGA)
Center for PlantConservation (CPC) – Conservation officer