Anthriscus cerefolium Inhibits growth of Gram-negative Bacteria
Chervil (Anthriscus cerefolium) is a culinary herb in the Apiaceae family that is native to Caucasus. It has been used medically as a skin lotion and eyewash. Finding antimicrobial properties in a Chervil extract is important because is could potentially lead to improved means of creating and collecting antibiotic substances to be used for medicinal purposes. This would be of great use because it would add an antimicrobial substance to the list of antibiotics used to treat gram-negative bacterial infections. As the number of antibiotic resistant microbes increases, the need for new antimicrobial substances becomes increasingly important in combating the mutated microbes that have become resistant to other antibiotics. Foodborne E. coli associated with fresh produce infects………In our experiment, we will be looking for the inhibited growth of gram-negative bacteria in relation to our A. cerefolium extractThe purpose of our study is…determine whether this herb might kill….
Chervil leaves were ground at room temperature in sterile water, 70% ethanol, and 70% methanol to a final concentration of 0.1 100 mg/mL extract. The alcoholic extracts were tested against four microorganisms, three gram-positive bacteria ( Bacillus subtilis, Staphylococcus aureus, Streptococcus pyogenes) and one gram -negative bacteria (Escherichia coli) . Resutls were taken after 24 hours of incubation.A Controls that showed inhibition of bacterial growth included the following combinations; Acetone control and S. pyogenes, H2O control and B. subtilis, H2O control and E.coli, and H2O control with S. Pyogenes. These controls inhibited bacterial growth negating the results of the chervil extracts for those trials. The Chervil extracts that were successful in inhibiting bacterial growth included Methyl and Ethyl alcoholic chervil extracts in combination withinhibited Escherichia coli, and S. aureus. The Minimal minimum inhibitory concentration for both methyl methanolic and ethyl ethanolic extracts was 0.003.1325 mg/ml concentration against Escherichia coli and S. aureus????? . We will be testing a chervil extract for gram-negative bacterial inhibition in bean sprouts. In June of 2011, thousands of people were infected in Germany with E. coli contaminated bean sprouts in which 31 died. A. cerefolium has antibacterial properties that might be used to prevent the growth of gram-negative bacteria. We will screen our chervil extracts in a bean sprout suspension with the expectation of E.coli growth inhibition. We will then conduct an MIC determination to find the minimal concentration of chervil extract needed to inhibit E. coli growth. Then we will cunduct a population growth experiment using spectrophotometry to to determine a bacterial growth curve in relation to our chervil extract in a bean sprout suspension. Valid data from these experiments will provide evidence that chervil might be used to limit the number of E. coli outbreaks in fresh foods.What is in progress? New results?